LFA App User's Guide
Introduction | Testing our apps without installing | Installation | Start Apps | Tab 1: Cropping and Segmentation | Upload Image or Choose Sample Image | Set number of strips and number of lines per strip | Apply Segmentation | Tab 2: Background | Select Strip | Optional: in case of color images | Select threshold method and apply | Add to intensity data | Switch To Intensity Data | Tab 3: Intensity Data | Download data | Restart with new data | Upload existing data | Switch To Experiment Info | Tab 4: Experiment Info | Upload Experiment Info or upload existing merged data and go to calibration | Select ID columns and merge datasets | Prepare Calibration | Tab 5: Calibration | Specify a folder for the analysis results | Upload existing preprocessed data | Optional: average technical replicates | Optional: reshape data from long to wide | Calibration by linear, local polynomial or generalized additive model | Run calibration analysis | Tab 6: Results | Open analysis report | Tab 7: Quantification | Required R packages | References